4M8Q
Ontogeny of recognition specificity and functionality for the anti-HIV neutralizing antibody 4E10
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-04-17 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 72.561, 77.674, 77.823 |
Unit cell angles | 90.00, 112.03, 90.00 |
Refinement procedure
Resolution | 24.610 - 2.890 |
R-factor | 0.26294 |
Rwork | 0.261 |
R-free | 0.30168 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.733 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.610 | 2.950 |
High resolution limit [Å] | 2.890 | 2.890 |
Number of reflections | 17941 | |
Completeness [%] | 99.6 | 97.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.1M KCl, 12% w/v PEG 8000, 5% v/v glycerol, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |