4M78
Crystal structure of Lsm2-8 complex, space group P21
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-08 |
| Detector | RAYONIX MX325HE |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.721, 76.638, 113.554 |
| Unit cell angles | 90.00, 97.00, 90.00 |
Refinement procedure
| Resolution | 29.068 - 2.794 |
| R-factor | 0.2555 |
| Rwork | 0.254 |
| R-free | 0.29360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4m75 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.376 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.794 | 2.794 |
| Number of reflections | 33539 | |
| Completeness [%] | 98.2 | 83 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 100mM sodium cacodylate 6.5, 25% Jeffamine ED2001, 20mM BaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
