4M74

Mutant structure of methyltransferase from Streptomyces hygroscopicus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-08-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	57.391, 89.916, 137.559
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.830 - 2.200
R-factor	0.16842
Rwork	0.165
R-free	0.23408
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4kib
RMSD bond length	0.015
RMSD bond angle	1.727
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER (MR)
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.240
High resolution limit [Å]	2.200	2.200
Number of reflections	31111
Completeness [%]	99.9	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	16% PEG3350, 0.2M sodium iodine, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K