4M74
Mutant structure of methyltransferase from Streptomyces hygroscopicus
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Methyltransferase MppJ | polymer | 357 | 39342.2 | 2 | UniProt (Q643C8) | Streptomyces hygroscopicus | |
| 2 | C, J (A, B) | S-ADENOSYL-L-HOMOCYSTEINE | non-polymer | 384.4 | 2 | Chemie (SAH) PubChem (193) PubChem (13792) PubChem (189149) PubChem (439155) PubChem (5284331) PubChem (71170554) PubChem (13231890) PubChem (16760024) PubChem (25210495) PubChem (44361184) PubChem (177678424) PubChem (46936206) PubChem (21125603) PubChem (25203782) PubChem (52224400) PubChem (50989525) PubChem (51380914) PubChem (52224398) PubChem (6419189) PubChem (57544532) PubChem (59046843) PubChem (59170628) PubChem (164157219) PubChem (59576228) PubChem (66805708) PubChem (70689219) PubChem (70702054) PubChem (70832431) PubChem (118374891) PubChem (118560068) PubChem (126969526) PubChem (129105892) PubChem (143518505) PubChem (154578335) PubChem (163054214) PubChem (163335525) | |||
| 3 | D, K (A, B) | (2S,3R)-2,3-dihydroxy-3-phenylpropanoic acid | non-polymer | 182.2 | 2 | Chemie (M74) PubChem (265740) PubChem (737431) PubChem (737432) PubChem (737433) PubChem (737434) | |||
| 4 | E, L (A, B) | FE (III) ION | non-polymer | 55.8 | 2 | Chemie (FE) PubChem (29936) | |||
| 5 | F, M (A, B) | (2R)-2-hydroxy-3-phenylpropanoic acid | non-polymer | 166.2 | 2 | Chemie (HF2) | |||
| 6 | G, H, I, N (A, B) | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) PubChem (271) | |||
| 7 | O, P (A, B) | water | water | 18.0 | 496 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 337 (UniProt: Q643C8)
| PDB | External Database | Details |
|---|---|---|
| Met -19 | - | expression tag |
| Gly -18 | - | expression tag |
| Ser -17 | - | expression tag |
| Ser -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| Ser -9 | - | expression tag |
| Ser -8 | - | expression tag |
| Gly -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Val -5 | - | expression tag |
| Pro -4 | - | expression tag |
| Arg -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| His 0 | - | expression tag |
| Leu 127 | Arg 127 | engineered mutation |
| Ala 244 | Asp 244 | engineered mutation |
| Glu 300 | Val 300 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 78684.5 | |
| Non-Polymers* | Number of molecules | 12 |
| Total formula weight | 1737.5 | |
| All* | Total formula weight | 80422.0 |
