4M6R
Structural and biochemical basis for the inhibition of cell death by APIP, a methionine salvage enzyme
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.00000, 0.91977, 0.91957, 0.90633 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 107.035, 107.735, 192.099 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.000 |
R-factor | 0.18294 |
Rwork | 0.181 |
R-free | 0.22027 |
Structure solution method | MAD |
RMSD bond length | 0.026 |
RMSD bond angle | 2.049 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 96.050 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 72069 | |
Completeness [%] | 96.0 | 81.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 285 | 0.04M citric acid, 0.06M bis-tris propane, 5%(v/v) glycerol, 21%(w/v) PEG 3350, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 285K |