4M6O

Crystal structure of anti-NGF antibody CNTO7309

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	95
Detector technology	CCD
Collection date	2009-08-10
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5418
Spacegroup name	I 2 3
Unit cell lengths	152.600, 152.600, 152.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.800
R-factor	0.18877
Rwork	0.186
R-free	0.25047
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1VGE AND 2AAB
RMSD bond length	0.007
RMSD bond angle	1.342
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.086	0.405
Number of reflections	13878
<I/σ(I)>	14.5	1.8
Completeness [%]	95.7	61.7
Redundancy	3.4	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1 M MES, pH 6.5, 12% PEG20000, VAPOR DIFFUSION, SITTING DROP, temperature 293K