4M5X
Crystal structure of the USP7/HAUSP catalytic domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2011-12-12 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.951, 67.579, 76.805 |
| Unit cell angles | 90.00, 96.21, 90.00 |
Refinement procedure
| Resolution | 38.177 - 2.187 |
| R-factor | 0.1821 |
| Rwork | 0.180 |
| R-free | 0.22140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nb8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.161 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX (1.8.1_1168) |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.354 | 2.240 |
| High resolution limit [Å] | 2.187 | 2.187 |
| Rmerge | 0.077 | 0.508 |
| Number of reflections | 38925 | |
| <I/σ(I)> | 20.44 | 1.9 |
| Completeness [%] | 98.5 | 89 |
| Redundancy | 3.4 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 285 | 0.1 M HEPES, pH 7.5, 22% PEG3350, 0.2 M sodium bromide, 0.15 mM Cymal-7, VAPOR DIFFUSION, SITTING DROP, temperature 285K |
