4M2Z

Crystal structure of RNASE III complexed with double-stranded RNA and CMP (TYPE II CLEAVAGE)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2006-03-08
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 32 2 1
Unit cell lengths	81.131, 81.131, 223.892
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.110 - 2.850
R-factor	0.2244
Rwork	0.221
R-free	0.28870
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.003
RMSD bond angle	0.698
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.950
High resolution limit [Å]	2.850	6.120	2.850
Rmerge	0.064	0.045	0.304
<I/σ(I)>	23.1	25.97	1.86
Completeness [%]	89.9	86.8	75.2
Redundancy	4.6	5.5	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	70% (v/v) MPD in 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K