4M0U

crystal structure of human PRS1 Q133P mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Spacegroup name	H 3
Unit cell lengths	169.880, 169.880, 61.764
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.050 - 2.740
R-factor	0.25539
Rwork	0.253
R-free	0.29772
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4f8e
RMSD bond length	0.010
RMSD bond angle	1.159
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.050
High resolution limit [Å]	2.740	2.740
Number of reflections	16179

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.1	295	pH 4.1, VAPOR DIFFUSION, temperature 295K