4LZZ

Nucleotide-induced asymmetry within atpase activator ring drives s54-RNAP interaction and ATP hydrolysis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-01-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	119.970, 130.830, 208.230
Unit cell angles	90.01, 90.01, 89.85

Refinement procedure

Resolution	44.269 - 3.210
R-factor	0.2676
Rwork	0.267
R-free	0.32230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.127
Data reduction software	d*TREK (9.7 W8RSSI)
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.270	44.270	2.970
High resolution limit [Å]	2.870	6.180	2.870
Rmerge	0.118	0.032	0.511
Number of reflections	256925
<I/σ(I)>	3	12.1	0.8
Completeness [%]	88.7	90.3	76.1
Redundancy	1.76	1.81	1.62

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.9	277	20% ethylene glycol, crystal soaked in fresh mother liquor prior to flash cooling, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 277K