4LZB
Uracil binding pocket in Vaccinia virus uracil DNA glycosylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-13 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.77 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 93.470, 114.045, 302.516 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.640 - 2.030 |
| R-factor | 0.21455 |
| Rwork | 0.213 |
| R-free | 0.24607 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4DOF CHAIN A |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.049 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.640 | 2.140 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmerge | 0.130 | 0.584 |
| Number of reflections | 198856 | |
| <I/σ(I)> | 25.9 | 3.9 |
| Completeness [%] | 95.2 | 88.9 |
| Redundancy | 17 | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 10% PEG8000, 10% DMSO, 0.1 M Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
