4LXJ
Saccharomyces cerevisiae lanosterol 14-alpha demethylase with lanosterol bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.213, 67.005, 80.378 |
| Unit cell angles | 90.00, 99.54, 90.00 |
Refinement procedure
| Resolution | 29.327 - 1.900 |
| R-factor | 0.1966 |
| Rwork | 0.195 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ld6 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.423 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.045 | |
| Number of reflections | 62523 | |
| Completeness [%] | 96.5 | 72.8 |
| Redundancy | 3.5 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 9 | 291 | 49% PEG 400, 100mM Glycine pH 9.0, VAPOR DIFFUSION, temperature 291K |
