4LWV
The 2.3A Crystal Structure of Humanized Xenopus MDM2 with RO5545353
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-15 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.99990 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 88.742, 132.093, 35.797 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.440 - 2.320 |
| R-factor | 0.233 |
| Rwork | 0.230 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.800 |
| Data reduction software | CrystalClear |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNX (2005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.020 | 2.450 |
| High resolution limit [Å] | 2.320 | 2.320 |
| Rmerge | 0.122 | |
| Number of reflections | 19022 | |
| <I/σ(I)> | 11 | |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 278 | 40-50% Saturated Ammonium Sulfate, 0.1M MES, pH 6.5, 5% PEG200, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
