4LT6

Crystal Structure of human poly(A) polymerase gamma

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-03-19
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	68.870, 89.920, 201.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.884 - 2.790
R-factor	0.1962
Rwork	0.193
R-free	0.25740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1q78
RMSD bond length	0.009
RMSD bond angle	1.250
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.6_289))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	2.970
High resolution limit [Å]	2.790	5.060	2.790
Number of reflections	31418
Completeness [%]	98.1	99.7	89.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	20%(W/V) PEG 8000, 50mM Tris-HCl, 100mM (NH4)2SO4, 5mM CaCl2, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K