4LSG

Structure of Mouse P-Glycoprotein

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2007-12-16
Detector	MARMOSAIC 300
Wavelength(s)	1.00695, 1.00923
Spacegroup name	P 21 21 21
Unit cell lengths	97.542, 115.426, 378.858
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.983 - 3.800
R-factor	0.3378
Rwork	0.336
R-free	0.35690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ksb
RMSD bond length	0.006
RMSD bond angle	1.309
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.8.2_1309)
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	4.040
High resolution limit [Å]	3.800	3.800
Number of reflections	41131
Completeness [%]	96.1	86.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	278	17% PEG 350mme, 0.05M tris, 0.04% sodium cholate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 278K