4LSA
Crystal structure of BRI1 sud1 (Gly643Glu) bound to brassinolide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979630 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 174.033, 67.303, 120.901 |
| Unit cell angles | 90.00, 121.09, 90.00 |
Refinement procedure
| Resolution | 49.557 - 2.500 |
| R-factor | 0.2075 |
| Rwork | 0.205 |
| R-free | 0.25060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rj0 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.742 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1334)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.560 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.055 | 0.967 |
| Number of reflections | 41658 | |
| <I/σ(I)> | 20.74 | 1.81 |
| Completeness [%] | 99.2 | 88.5 |
| Redundancy | 6.75 | 6.08 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | 23% PEG4000, 0.2 M ammonium sulfate, 0.1 M citric acid, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
