4LRM
EGFR D770_N771insNPG in complex with PD168393
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-07-16 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 114.923, 425.845, 95.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.181 - 3.526 |
R-factor | 0.2269 |
Rwork | 0.225 |
R-free | 0.26380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2jiu |
RMSD bond length | 0.006 |
RMSD bond angle | 0.985 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.770 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.151 | 0.389 |
Number of reflections | 24856 | |
<I/σ(I)> | 5.6 | 1.7 |
Completeness [%] | 85.8 | 76.1 |
Redundancy | 2.3 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M Tris, 2% PEG400, 5mM tris(2-carboxyethyl)-phosphine (TCEP), pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |