4LQD

The crystal structures of the Brucella protein TcpB and the TLR adaptor protein TIRAP show structural differences in microbial TIR mimicry

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2010-03-25
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.033
Spacegroup name	P 43 21 2
Unit cell lengths	87.570, 87.570, 80.650
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.960 - 2.451
R-factor	0.2079
Rwork	0.207
R-free	0.21820
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.978
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	10.960
High resolution limit [Å]	2.450
Number of reflections	12002
Completeness [%]	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	298	5%MPD,100mM Ches pH 9.0, 100mM NaCl and 24% glycerol cryo, VAPOR DIFFUSION, HANGING DROP, temperature 298K