4LNU

Nucleotide-free kinesin motor domain in complex with tubulin and a DARPin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-03-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 1
Unit cell lengths	73.400, 83.010, 83.307
Unit cell angles	116.16, 105.08, 97.57

Refinement procedure

Resolution	43.450 - 2.190
R-factor	0.1574
Rwork	0.155
R-free	0.19480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bg2 4drx
RMSD bond length	0.010
RMSD bond angle	1.170
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	BUSTER (2.10.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.450	2.310
High resolution limit [Å]	2.190	2.190
Number of reflections	77650
<I/σ(I)>	12.5	2.3
Completeness [%]	92.0	58.4
Redundancy	3.6	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	293	PEG, MES BUFFER, 0.2M AMMONIUM SULFATE, pH 6.50, VAPOR DIFFUSION, temperature 293K