4LN0

Crystal structure of the VGLL4-TEAD4 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-01-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	P 32 2 1
Unit cell lengths	94.817, 94.817, 135.172
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.057 - 2.896
R-factor	0.1918
Rwork	0.185
R-free	0.24710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3jua
RMSD bond length	0.009
RMSD bond angle	1.261
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.950
High resolution limit [Å]	2.896	2.900
Rmerge	0.164	0.942
Number of reflections	15590
<I/σ(I)>	15.86	5.28
Completeness [%]	96.5	97.6
Redundancy	5.5	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	289	0.2M L-proline, 0.1M HEPES pH 7.5, 24% PEG 1500, VAPOR DIFFUSION, SITTING DROP, temperature 289K