4LMF
C1s CUB1-EGF-CUB2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.876, 71.668, 157.723 |
| Unit cell angles | 90.00, 95.93, 90.00 |
Refinement procedure
| Resolution | 67.510 - 2.921 |
| R-factor | 0.2106 |
| Rwork | 0.208 |
| R-free | 0.25210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.773 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.510 | 3.000 |
| High resolution limit [Å] | 2.920 | 2.920 |
| Number of reflections | 32566 | |
| Completeness [%] | 98.9 | 91 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
