4LMB
Crystal structure analysis of O-acetylserine sulfhydrylase CysK2 complexed with cystine from Microcystis aeruginosa 7806
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-12-03 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97915 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 99.846, 99.846, 164.141 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.060 - 1.910 |
R-factor | 0.20308 |
Rwork | 0.202 |
R-free | 0.23028 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2q3b |
RMSD bond length | 0.009 |
RMSD bond angle | 1.304 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 1.910 | 2.300 |
Number of reflections | 36107 | |
Completeness [%] | 99.1 | 99.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 1.5M (NH4)2SO4, 0.1M Bis-Tris-propane, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |