4LLU
Structure of Pertuzumab Fab with light chain Clambda at 2.16A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2012-10-07 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | .97931 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 210.024, 69.739, 70.828 |
| Unit cell angles | 90.00, 98.75, 90.00 |
Refinement procedure
| Resolution | 19.900 - 2.160 |
| R-factor | 0.22501 |
| Rwork | 0.222 |
| R-free | 0.27279 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s78 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.328 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.902 | 2.280 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Rmerge | 0.175 | 0.670 |
| Number of reflections | 52049 | |
| Completeness [%] | 97.4 | 94.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 294 | 100mM Sodium Acetate pH 4.5 + 10% MPD + 30% PEG 2000 MME + 200mM Ammonium Sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
