4LLD
Structure of wild-type IgG1 antibody heavy chain constant domain 1 and light chain lambda constant domain (IgG1 CH1:Clambda) at 1.19A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 77 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-05-23 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | .97949 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.080, 65.837, 66.925 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.934 - 1.190 |
| R-factor | 0.14596 |
| Rwork | 0.145 |
| R-free | 0.16992 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3thm |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.286 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.925 | 1.250 |
| High resolution limit [Å] | 1.190 | 1.190 |
| Rmerge | 0.043 | 0.528 |
| Number of reflections | 60110 | |
| Completeness [%] | 97.6 | 95.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 294 | 35% PEG 4K, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
