4LJO
Structure of an active ligase (HOIP)/ubiquitin transfer complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-01-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.920 |
| Spacegroup name | P 31 |
| Unit cell lengths | 45.950, 45.950, 133.010 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.337 - 1.564 |
| R-factor | 0.1828 |
| Rwork | 0.181 |
| R-free | 0.21240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ljq |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.032 |
| Data reduction software | CCP4 |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.3.0) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 44.340 | 1.600 | |
| High resolution limit [Å] | 1.560 | 6.980 | 1.560 |
| Rmerge | 0.039 | 0.040 | 0.593 |
| Number of reflections | 44055 | ||
| <I/σ(I)> | 12.2 | 16.71 | 1.52 |
| Completeness [%] | 99.2 | 88.5 | 94 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293 | 0.1 M carboxylic acids, 0.1 M imidazole, MES, 30 % P550 MME_P20K, pH 6.5, vapour diffusion, temperature 293K, VAPOR DIFFUSION |
