4LIF

Crystal structure of the JCV large T-antigen origin binding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.075
Spacegroup name	I 41
Unit cell lengths	103.793, 103.793, 34.860
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.696 - 2.600
R-factor	0.1782
Rwork	0.176
R-free	0.22290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fuf
RMSD bond length	0.007
RMSD bond angle	0.990
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.600
Rmerge	0.068
Number of reflections	5906
<I/σ(I)>	37.6
Completeness [%]	99.8
Redundancy	14.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	273	0.1 M Tris, 0.2 M LiSO4, 30 % Peg 4000, VAPOR DIFFUSION, SITTING DROP, temperature 273K, pH 8.5