4LI0

Crystal structure of GDP-bound Rab8:GRAB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2009-05-01
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0000
Spacegroup name	C 2 2 21
Unit cell lengths	110.930, 160.380, 166.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.210 - 3.300
R-factor	0.24643
Rwork	0.244
R-free	0.28962
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yzq
RMSD bond length	0.010
RMSD bond angle	1.497
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	48.210
High resolution limit [Å]	3.200
Number of reflections	21124
Completeness [%]	99.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.3	293	1.6 M ammonium sulphate and sodium acetate. cryo solution containing 20% glycerol in the reservoir solution, pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K