4LH0
Structure of mouse 1-Pyrroline-5-Carboxylate Dehydrogenase (ALDH4A1) complexed with glyoxylate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 148 |
Detector technology | IMAGE PLATE |
Collection date | 2012-09-04 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 84.870, 94.242, 132.087 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.664 - 1.674 |
R-factor | 0.16 |
Rwork | 0.159 |
R-free | 0.18350 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.054 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.16) |
Phasing software | PHENIX |
Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 19.668 | 19.668 | 1.760 |
High resolution limit [Å] | 1.674 | 5.290 | 1.670 |
Rmerge | 0.043 | 0.234 | |
Total number of observations | 19061 | 57767 | |
Number of reflections | 120763 | ||
<I/σ(I)> | 14.9 | 11.8 | 3.2 |
Completeness [%] | 98.7 | 97.7 | 91.9 |
Redundancy | 4.5 | 4.7 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 15-25% PEG 3350, 0.2 M lithium sulfate, 0.1 M Bis-Tris, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |