4LFI
Crystal structure of scCK2 alpha in complex with GMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Wavelength(s) | 0.97906 |
| Spacegroup name | P 32 |
| Unit cell lengths | 69.664, 69.664, 166.251 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.860 - 1.850 |
| R-factor | 0.17202 |
| Rwork | 0.171 |
| R-free | 0.20046 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.266 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.850 |
| Number of reflections | 77066 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 287 | 0.2M Lithium sulfate monohydrate, 0.1M BIS-TRIS pH 6.5, 25% w/v PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 287K |
