4LF0

The E142D mutant of the amidase from Geobacillus pallidus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2013-05-05
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.7749
Spacegroup name	P 42 3 2
Unit cell lengths	130.633, 130.633, 130.633
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.330 - 1.100
R-factor	0.13112
Rwork	0.131
R-free	0.13749
RMSD bond length	0.025
RMSD bond angle	2.277
Data reduction software	XDS
Data scaling software	XDS
Phasing software	REFMAC
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.330	1.106
High resolution limit [Å]	1.100	1.101
Rmerge		0.401
Number of reflections	152140
<I/σ(I)>		12.58
Completeness [%]	99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1