4LDS
The inward-facing structure of the glucose transporter from Staphylococcus epidermidis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-04 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.8211 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 130.070, 118.850, 160.049 |
| Unit cell angles | 90.00, 100.08, 90.00 |
Refinement procedure
| Resolution | 19.902 - 3.200 |
| R-factor | 0.3038 |
| Rwork | 0.302 |
| R-free | 0.34120 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.057 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXS |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.280 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 38767 | |
| <I/σ(I)> | 7 | 1.9 |
| Completeness [%] | 97.2 | 91 |
| Redundancy | 4.8 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 24% PEG 400, 0.1 M Ca Acetate, 0.1 M NaCl, 0.1 M MES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
