4LDS
The inward-facing structure of the glucose transporter from Staphylococcus epidermidis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-06-04 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.8211 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 130.070, 118.850, 160.049 |
Unit cell angles | 90.00, 100.08, 90.00 |
Refinement procedure
Resolution | 19.902 - 3.200 |
R-factor | 0.3038 |
Rwork | 0.302 |
R-free | 0.34120 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 2.057 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHELXS |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.280 |
High resolution limit [Å] | 3.200 | 3.200 |
Number of reflections | 38767 | |
<I/σ(I)> | 7 | 1.9 |
Completeness [%] | 97.2 | 91 |
Redundancy | 4.8 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 24% PEG 400, 0.1 M Ca Acetate, 0.1 M NaCl, 0.1 M MES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |