4LAX
Crystal Structure Analysis of FKBP52, Complex with FK506
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.053, 41.870, 79.825 |
| Unit cell angles | 90.00, 101.55, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.007 |
| R-factor | 0.1853 |
| Rwork | 0.183 |
| R-free | 0.21890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q1c |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.353 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.5) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 78.326 | 47.836 | 2.110 |
| High resolution limit [Å] | 2.007 | 6.320 | 2.007 |
| Rmerge | 0.086 | 0.043 | 0.269 |
| Total number of observations | 1499 | 7096 | |
| Number of reflections | 19923 | ||
| <I/σ(I)> | 8 | 14.7 | 2.5 |
| Completeness [%] | 94.0 | 84.9 | 96.4 |
| Redundancy | 2.4 | 2.5 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 293 | 25% PEG6000, 0.1 M Tris-HCl, pH 8.0, 5% DMSO, VAPOR DIFFUSION, temperature 293K |
