4L5J
Crystal structures of the LsrR proteins complexed with phospho-AI-2 and its two different analogs reveal distinct mechanisms for ligand recognition
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-31 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.00000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 189.330, 80.611, 118.701 |
| Unit cell angles | 90.00, 118.01, 90.00 |
Refinement procedure
| Resolution | 47.320 - 2.600 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.320 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 45072 | |
| Completeness [%] | 78.4 | 92.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293 | 0.1 M Bis-Tris, 2% isopropanol, 8% gamma-butyrolactone, pH 6.5, VAPOR DIFFUSION, temperature 293K |
