4L44
Crystal structures of human p70S6K1-T389A (form II)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-02-26 |
Detector | CCD ADSC unsupported-q315 |
Wavelength(s) | 0.9797 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 77.961, 77.961, 113.829 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.900 |
R-factor | 0.2459 |
Rwork | 0.243 |
R-free | 0.29780 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.576 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.000 |
High resolution limit [Å] | 2.900 | 6.240 | 2.900 |
Rmerge | 0.134 | 0.093 | 0.376 |
Number of reflections | 9546 | ||
<I/σ(I)> | 9.7 | ||
Completeness [%] | 98.8 | 97.5 | 99.7 |
Redundancy | 3.9 | 3.6 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop | 7.2 | 289 | 2M lithium sulfate, 0.05M magnesium sulfate, 0.05M HEPES, pH 7.2, hanging drop, temperature 289K |