4L3T
Crystal Structure of Substrate-free Human Presequence Protease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 113.5 |
| Detector technology | CCD |
| Collection date | 2012-10-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97895 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 245.782, 85.093, 158.459 |
| Unit cell angles | 90.00, 127.54, 90.00 |
Refinement procedure
| Resolution | 42.547 - 2.030 |
| R-factor | 0.1726 |
| Rwork | 0.171 |
| R-free | 0.20980 |
| Structure solution method | MR/SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.988 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Number of reflections | 163827 | |
| <I/σ(I)> | 17.1 | 2.75 |
| Completeness [%] | 98.2 | 97 |
| Redundancy | 4.13 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291.15 | 15.2% w/v PEG8000, 15 mM TCEP, 80 mM sodium cacodylate, pH 6.5, 160 mM calcium acetate, 20% v/v glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K |
