4L17

GluA2-L483Y-A665C ligand-binding domain in complex with the antagonist DNQX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-05
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0332
Spacegroup name	P 2 21 21
Unit cell lengths	48.850, 96.790, 252.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.920 - 2.800
R-factor	0.1975
Rwork	0.195
R-free	0.24450
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.001
RMSD bond angle	0.429
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.1_357))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.924	2.950
High resolution limit [Å]	2.800	2.800
Number of reflections	29223
Completeness [%]	95.5	92.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277.15	19% PEG 1450, 0.3M lithium sulfate, 0.1M cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K