4KZN

crystal structure of human VEGF-A receptor binding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-04
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	87.573, 32.014, 42.981
Unit cell angles	90.00, 92.99, 90.00

Refinement procedure

Resolution	24.834 - 1.711
R-factor	0.182783276428
Rwork	0.179
R-free	0.21823
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdb 1vpf
RMSD bond length	0.012
RMSD bond angle	1.559
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (2.4.0)
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.770
High resolution limit [Å]	1.710	3.680	1.710
Rmerge	0.045	0.026	0.271
Number of reflections	12694
<I/σ(I)>	17.7
Completeness [%]	97.4	95.8	96.4
Redundancy	3.5	3.3	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	295	Li2SO4, PEG200, Tris buffer, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K
1	VAPOR DIFFUSION, SITTING DROP	7	295	Li2SO4, PEG200, Tris buffer, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K