4KXQ

Structure of NAD-dependent protein deacetylase sirtuin-1 (closed state, 1.85 A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-04-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.28210
Spacegroup name	P 32 2 1
Unit cell lengths	92.714, 92.714, 97.745
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.357 - 1.849
R-factor	0.1695
Rwork	0.168
R-free	0.18940
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.227
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELX
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.849	1.849
Number of reflections	41954

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.4		100 mM Tris, pH 7.4, 13% PEG20000