4KXM
Crystal structure of DNPH1 (RCL) WITH N6-ISOPENTENYL-AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Detector technology | CCD |
| Collection date | 2010-11-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979756 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.146, 96.568, 79.034 |
| Unit cell angles | 90.00, 101.58, 90.00 |
Refinement procedure
| Resolution | 38.710 - 2.240 |
| R-factor | 0.1765 |
| Rwork | 0.174 |
| R-free | 0.22720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fyi |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.146 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.970 | 2.390 |
| High resolution limit [Å] | 2.240 | 2.240 |
| Rmerge | 0.050 | 0.107 |
| Number of reflections | 21068 | |
| <I/σ(I)> | 11.1 | 6.7 |
| Completeness [%] | 92.0 | 65.4 |
| Redundancy | 3.4 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 18% PEG8K, 100 MM NaCaCo, 200 MM ZINC ACETATE, PH 6.5, vapor diffusion, hanging drop, temperature 291K |
