4KXK
Alanine-glyoxylate aminotransferase variant K390A/K391A in complex with the TPR domain of human Pex5p
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-27 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.812 |
| Spacegroup name | P 1 |
| Unit cell lengths | 57.593, 74.698, 91.209 |
| Unit cell angles | 87.85, 83.62, 89.79 |
Refinement procedure
| Resolution | 19.800 - 2.900 |
| R-factor | 0.18678 |
| Rwork | 0.184 |
| R-free | 0.23764 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3r9a |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.390 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 19.800 |
| High resolution limit [Å] | 2.900 |
| Rmerge | 0.134 |
| Number of reflections | 32399 |
| Redundancy | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 293 | 0.1 M Bis-Tris (pH 5.3), 0.10 M LiSO4, 12 % [w/w] PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
