4KXF

Crystal structure of NLRC4 reveals its autoinhibition mechanism

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	200
Detector technology	CCD
Collection date	2012-12-10
Detector	ADSC QUANTUM 210r
Wavelength(s)	1.0, 1.008, 1.06, 1.225
Spacegroup name	P 41 21 2
Unit cell lengths	334.052, 334.052, 177.567
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.326 - 3.200
R-factor	0.228
Rwork	0.226
R-free	0.26560
Structure solution method	MAD
RMSD bond length	0.004
RMSD bond angle	1.108
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	3.310
High resolution limit [Å]	3.200	3.200
Number of reflections	150190
Completeness [%]	90.9	92.2
Redundancy	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.9	291	1.65M sodium phosphate monobasic monohydrate/potassium phosphate dibasic, 1% n-propyl formate., pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 291K