4KV5
scFv GC1009 in complex with TGF-beta1.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-15 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 108.188, 171.769, 109.876 |
| Unit cell angles | 90.00, 111.30, 90.00 |
Refinement procedure
| Resolution | 30.960 - 3.000 |
| R-factor | 0.2329 |
| Rwork | 0.229 |
| R-free | 0.30030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.384 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.960 | 30.960 | 3.160 |
| High resolution limit [Å] | 3.000 | 6.550 | 3.050 |
| Rmerge | 0.059 | 0.467 | |
| Number of reflections | 36020 | ||
| <I/σ(I)> | 14.1 | 1.9 | |
| Completeness [%] | 99.8 | 98.9 | 99.8 |
| Redundancy | 3.2 | 3.38 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 294 | 16% PEG 4K, 0.1 M citrate, 4% 2-propanol , pH 5.0, VAPOR DIFFUSION, temperature 294K |
