4KTY
Fibrin-stabilizing factor with a bound ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 1 |
| Unit cell lengths | 56.761, 80.510, 102.844 |
| Unit cell angles | 88.14, 76.70, 82.01 |
Refinement procedure
| Resolution | 20.795 - 1.980 |
| R-factor | 0.171 |
| Rwork | 0.169 |
| R-free | 0.20640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f13 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.366 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Number of reflections | 119138 | |
| <I/σ(I)> | 10 | 2 |
| Completeness [%] | 97.1 | 95.8 |
| Redundancy | 2.2 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 170 mM ammonium sulfate 85 mM sodium cacodylate 25.5 % PEG8000 15 % glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
