4KSL

Gumby/Fam105B in complex with linear di-ubiquitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-02-27
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 2
Unit cell lengths	183.622, 186.087, 219.698
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.830
R-factor	0.226
Rwork	0.224
R-free	0.25400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	GUMBY/FAM105B UBIQUITIN
RMSD bond length	0.004
RMSD bond angle	0.979
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.830	2.800
Number of reflections	173426
Completeness [%]	99.9	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.5	298	100mM acetate, 100mM CaCl2, 100 mM glycine, 2.5M sodium formate, 24% PEG3350 , pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K