4KPS

Structure and receptor binding specificity of the hemagglutinin H13 from avian influenza A virus H13N6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-03-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97922
Spacegroup name	P 1
Unit cell lengths	75.638, 76.804, 76.380
Unit cell angles	85.60, 82.67, 87.52

Refinement procedure

Resolution	42.455 - 2.587
R-factor	0.2181
Rwork	0.216
R-free	0.25480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4f23
RMSD bond length	0.005
RMSD bond angle	0.896
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.587	2.600
Number of reflections	50525
Completeness [%]	95.7	97.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2	293	0.2M L-Proline, 0.1M HEPES, 10% PEG-3350, 0.01M Sodium Bromide, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K