4KP4
Deciphering cis-trans directionality and visualizing autophosphorylation in histidine kinases.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-27 |
| Detector | PSI PILATUS 6M |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 163.686, 163.686, 130.093 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.580 - 3.000 |
| R-factor | 0.196 |
| Rwork | 0.194 |
| R-free | 0.23400 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.286 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.580 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.074 | 0.939 |
| Number of reflections | 20516 | |
| <I/σ(I)> | 2.3 | |
| Completeness [%] | 98.5 | |
| Redundancy | 10.6 | 10.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 294 | 2% PEG 1000, 2% PEG 4000, 0.03M sodium acetate, Hepes pH 7.5, ammonium sulfate 1.5M, VAPOR DIFFUSION, SITTING DROP, temperature 294K | |
| 1 | 7.5 | 294 | 2% PEG 1000, 2% PEG 4000, 0.03M sodium acetate, Hepes pH 7.5, ammonium sulfate 1.5M, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
