4KP3
Crystal Structure of MyoVa-GTD in Complex with Two Cargos
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Collection date | 2012-12-15 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.850, 107.933, 83.510 |
| Unit cell angles | 90.00, 96.14, 90.00 |
Refinement procedure
| Resolution | 38.694 - 2.405 |
| R-factor | 0.1945 |
| Rwork | 0.192 |
| R-free | 0.24110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wb8 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.769 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.067 |
| Number of reflections | 54968 |
| <I/σ(I)> | 24.7 |
| Completeness [%] | 98.4 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 12-16%(w/v) PEG3350, 2-4% tacsimate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
