4KNG

Crystal structure of human LGR5-RSPO1-RNF43

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-15
Detector	MARRESEARCH
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	104.575, 120.971, 181.009
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.510 - 2.500
R-factor	0.23425
Rwork	0.232
R-free	0.27633
Structure solution method	SIRAS
RMSD bond length	0.013
RMSD bond angle	1.844
Data reduction software	HKL-3000
Data scaling software	SCALA
Phasing software	X-PLOR
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall
Low resolution limit [Å]	39.510
High resolution limit [Å]	2.500
Completeness [%]	97.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	10% PEG4000, 0.2M ammonium sulfate, 7% Sucrose and 0.1 M TRIS pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K