4KNF
Crystal structure of a blue-light absorbing proteorhodopsin double-mutant D97N/Q105L from HOT75
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Collection date | 2011-10-01 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 161.400, 168.900, 65.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.950 - 2.600 |
| R-factor | 0.22173 |
| Rwork | 0.219 |
| R-free | 0.26654 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID: 4KLY |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.595 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.970 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.105 | 1.120 |
| Number of reflections | 48405 | |
| <I/σ(I)> | 11.28 | 1.76 |
| Completeness [%] | 98.5 | 98.6 |
| Redundancy | 7.06 | 7.08 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 20 mg/mL protein mixed with equal volume of reservoir solution (0.2 M ammonium acetate, 0.1 M bis-tris pH 6.5, 45%(v/v) MPD), VAPOR DIFFUSION, HANGING DROP, temperature 295K |
