4KLS
DNA polymerase beta mismatched reactant complex with Mn2+, 10 min
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-25 |
| Detector | RIGAKU SATURN 92 |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.920, 78.607, 55.034 |
| Unit cell angles | 90.00, 112.88, 90.00 |
Refinement procedure
| Resolution | 24.128 - 1.978 |
| R-factor | 0.2292 |
| Rwork | 0.226 |
| R-free | 0.28530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3c2m |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.229 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.7_650) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.050 |
| High resolution limit [Å] | 1.978 | 1.978 |
| Rmerge | 0.078 | 0.418 |
| Number of reflections | 30106 | |
| <I/σ(I)> | 14.3 | 2.45 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 4.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 291 | 50 mM imidazole, 350 mM sodium chloride, 17% PEG3350, pH 8.0, VAPOR DIFFUSION, temperature 291K |
